Author Topic: TB binding site binding affinity compared to mutant  (Read 70 times)

amanzour

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TB binding site binding affinity compared to mutant
« on: September 17, 2019, 10:48:43 pm »
Hi
How can I calculate the change between binding affinity of a transcription factor binding site and its corresponding mutated version of it. TF protein is known.
Would it be possible to do it web based?
Basically inputting: Sequence, mutant, TF and outputting change in TF binding affinity

Thank you so much beforehand

xiangjun

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Re: TB binding site binding affinity compared to mutant
« Reply #1 on: September 18, 2019, 09:36:42 pm »
Thanks for using the REDUCE Suite and for posting your questions on the Forum.

Unfortunately, I do not know how to calculate the change in binding affinity between wild and mutated sites of a TF binding site. Currently, the REDUCE Suite is command-line based only, no web interface to it.

Best regards,

Xiang-Jun

amanzour

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Re: TB binding site binding affinity compared to mutant
« Reply #2 on: October 01, 2019, 05:16:41 pm »
Thank you Lu.

How can I install Reducesuite?
are there a series of command line for linux?
Thank you
Amir

xiangjun

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Re: TB binding site binding affinity compared to mutant
« Reply #3 on: October 01, 2019, 09:16:05 pm »
Did you read the Documentation Section?

 

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